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Extracting DNA from frozen plasma

Drrutledge drrutledge at aol.com
Sat Sep 9 07:59:04 EST 1995


Are there any special precautions about that need to be taken when thawing
out a blood sample (plasma) and then extracting DNA?  Since the sample was
frozen, I suspect that all the cells will be broken (from being frozen and
thawed) and I thought that this could affect the high molecular weight DNA
(due to DNAses..?) which could be a problem during a PCR reaction. Help.



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