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Smeared PCR products

Mark Siddall mes at zoo.toronto.edu
Fri Feb 24 00:40:59 EST 1995


Smeared products frequently result from too much DNA template.
A good indication of this is if you use up the primers (i.e., 
can't see 'em at the end).

Do a quick template determination.
If this doesn't work try adding 10% DMSO to the reaction.

Mark

-- 
Mark E. Siddall                "I don't mind a parasite...
mes at vims.edu                    I object to a cut-rate one" 
Virginia Inst. Marine Sci.                     - Rick
Gloucester Point, VA, 23062



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