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anyone working with REM?

croyleml at ucbeh.san.uc.edu croyleml at ucbeh.san.uc.edu
Wed May 26 20:00:02 EST 1993


"Recursive Ensemble Mutagenesis (REM) is a algorithm which enhances the
frequency of 
functional mutants in a library when an appropriate selection or screening
method is 
employed.  REM uses successive rounds of Combinatorial Cassette Mutagenesis
(CCM)
to generate a diverse library of genetically altered proteins that fit a
certain selection 
criteria.  Amino acids are retained in the library if they are found in an
altered protein fitting the 
selection criteria.  Lists of all amino acids that are acceptable at each
mutated position
(i.e. 'target sets' of amino acids) are compiled.  In the next iteration of REM
combinatorial 
cassettes are resynthesized according to mathematical functions that bias the
nucleotide
mixtures at each mutated position in the protein to encode these target sets of
amino acids."



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