Does anyone have suggestions for PCR primers which would be effective
for amplifying the small subunit ribosomal (16S) gene of archea. I have
one that corresponds to the 1510-1492 region in E. coli (apparently a
'universal' site), but I need another for the 5' end of the gene. Also,
would there be any problems if I constructed it with a Not I polylinker on
the end for easier cloning? Thanks in advance.