Strange things started happening in this lab when RAPD analysis entered.
Promising at first, I find it hard now to manipulate single RAPD
fragments. Can they be cloned> Can they reproducibly be generated
by any lot# of Taq polymerase? So far I have had no succes in any
cloning procedure including T4, Klenow and kinase treatment.
Recognize some of these problems? Suggestions? Like to hear them.
L. v.d. Zande
Dept of genetics
University of Groningen