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Sequencing: Both strands or one?

Bruce Roe broe at aardvark.ucs.uoknor.edu
Tue Jun 16 06:32:00 EST 1992


In article <1992Jun15.185115.19849 at magnus.acs.ohio-state.edu>, rrumpf at magnus.acs.ohio-state.edu (Robert Rumpf) writes...
>I have a question, more of a survey, really, for anyone doing phylogeny 
>studies.  Are you sequencing both strands of whatever gene you are sequencing, 
>or just one and assume that's good enough?
> 
>Bob

To insure accuracy of each base, the "rule of three" should be followed.
Sequence each base twice in one orientation and once in the other, as a
minimum.  I can think of no instance, except for sloppy science, that this
rule should not be followed.

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