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Sequencing: Both strands or one?

Francis Ouellette francis at monod.biol.mcgill.ca
Tue Jun 16 08:22:04 EST 1992


rrumpf at magnus.acs.ohio-state.edu (Robert Rumpf) writes...

>>I have a question, more of a survey, really, for anyone doing phylogeny 
>>studies.  Are you sequencing both strands of whatever gene you are sequencing, 
>>or just one and assume that's good enough?
>> 
>>Bob
>

 broe at aardvark.ucs.uoknor.edu (Bruce Roe) writes:

>To insure accuracy of each base, the "rule of three" should be followed.
>Sequence each base twice in one orientation and once in the other, as a
>minimum.  I can think of no instance, except for sloppy science, that this
>rule should not be followed.
>


Bruce,

Is that what they do with the human cDNAs ...   ???   :-j


francis


---
| B.F. Francis Ouellette  
| manager, yeast chromosome I project
| dept of biology, McGill university, Montreal, Qc, Canada
| francis at monod.biol.mcgill.ca



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