Indiana University Offers
Recombinant DNA Laboratory Courses
During the summer of 1999, Indiana University's Department of Biology,
in
cooperation with the I.U. Division of Continuing Studies, will offer two
week-long laboratory courses focusing on the techniques and procedures
used
in recombinant DNA research and their application. Participants also
have
the opportunity to work with a DNA sample of their own research
organism,
if they choose. Both courses will be taught on the Indiana University
campus in Bloomington.
Recombinant DNA Technology
The first course, "Recombinant DNA Technology," will introduce
participants
to procedures involved in recombinant DNA work and to the molecular
aspects
of genetic engineering. Most of the procedures that are taught to
biology
graduate students in the recombinant DNA section of a graduate
techniques
course at Indiana University will be covered. Participants can make
arrangements to isolate genomic DNA from their own research organisms
during the course.
The following techniques will be included:
Participants in this course learn the following techniques:
o DNA and cloning vector preparation
o Preparation of recombinant DNA
o Transformation of bacterial cells
o Transformation of yeast cells
o Selection and assay of cloned and amplified fragments of "foreign"
DNA
o Analysis of signal transducing pathway using yeast two hybrid system
o PCR technology
o Southern blot analysis
o Preparation of nonradioactive DNA probes
o Use of web sites in research and teaching
"Recombinant DNA Technology" is designed for those with a basic
understanding of the structure of DNA and elemental genetics and with a
minimal understanding of enzymes and biochemistry.
The course is scheduled for June 13-18, 1999. Registration deadline is
May 14.
Application of Recombinant DNA Technology:
RFLP and Fingerprinting Analysis,
RAPD Analysis, and DNA Sequencing
This course will provide participants with the opportunity to learn
about
the materials and techniques used in recombinant DNA research.
Participants
may bring a DNA sample to sequence during the course.
This course will emphasize the following techniques:
o RAPD analysis of genomic DNA
o Fingerprinting and RFLP analysis of genomic DNA
o Chemiluminescent detection of nucleic acids
o Electroporation of bacterial cells
o DNA sequencing
o Preparation of random fragment sequencing libraries and
double-stranded DNA for sequencing
o Use of the BioNeb cell and biopolymer disruption system
o DNA sequencing using an automatic DNA sequencer
o Application of computers to DNA sequencing data analysis
o Use of web sites for molecular biology
The course is designed for people with a basic understanding of the
structure of DNA and a minimal understanding of enzymes and
biochemistry.
Previous experience with PCR analysis, RFLP analysis, and DNA sequencing
is
not necessary
This course is scheduled for June 20-25, 1999. Registration deadline
is
May 14.
The instructor for both courses is Dr. Stefan J. Surzycki, associate
professor of biology at Indiana University.
The registration fee for each course is $890.00. The fee for those
enrolling in both courses is $1,600.00. The fees include all
instruction,
laboratory supplies, use of equipment, and lab manuals.
For additional information, contact Jane Clay, Bloomington Division of
Continuing Studies, Indiana University, Owen Hall 204, Bloomington, IN
47405, phone (812) 855-6329.
Internet: JClay at Indiana.edu
Web: http://www.indiana.edu/~scs/iub/DNA.html
Jane Clay
Division of Continuing Studies
Owen Hall 204
Indiana University
Bloomington Indiana 47405
(812) 855-6329
