I have used D5S392 in the past with no problems (using the same primers as
Fleming described). I am however using 32P to detect the product.
My PCR conditions are
94C 60 sec
60 90 } 10 cycles
72 90
94C 60 sec
55 90 } 25 cycles
72 90
Mg = 1.5mM
I know the length of the cycles is a bit of an overkill but I use these
conditions to amplify all my AC repeats. I'm not sure if these conditions
will be suitable for fluorescent detection though.
Robyn
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Robyn Wallace
Department of Cytogenetics and Molecular Genetics
Womens and Childrens Hospital
North Adelaide, South Australia 5006
Ph 61 08 204 6442
Fax 61 08 204 7342
Email rwallace at mad.adelaide.edu.au
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