Gene Expression and Mutation Analysis
Holiday Inn, Foster City, CA
April 16th, 1996 (Tuesday)
[Sponsored by the Californian Separation Science Society]
8:45 - 9:00 am Gene expression and mutation analysis - a perspective.
To be named
Quantitation of Gene Expression
9:00 - 9:30 am Development of branched DNA (bDNA) signal amplification
assays for the direct quantitation of gene expression.
Janice A. Kolberg
Nucleic Acid Systems, Chiron Corp., Emeryville, CA
9:30 - 10:00 am Accurate quantitation of gene expression by single-tube
competitive RT-PCR and HPLC.
Peter A. Doris
Department of Cell Biology and Biochemistry,
Texas Tech University, Lubbock, TX
10:00 - 10:30 am Characterization of gene expression in single neurons by
patch-clamp and single-cell RT-PCR.
Lillian W. Chiang
Department of Neurobiology, Stanford University, CA
10:30 - 11:00 am Coffee Break
Gene Expression and Function Analysis
11:00 - 11:30 am Parallel gene expression monitoring on high-density cDNA
microarrays.
Mark Schena
Department of Biochemistry, Stanford University, CA
11:30 - 12:00 am High-throughput functional analysis of open reading
frames using tagged deletion strains and high-density
oligonucleotide arrays.
Dan Shoemaker
Department of Biochemistry, Stanford University, CA
12:00 - 12:30 am Genomic Analysis of Gene Function in Yeast using Genetic
Footprinting.
Victoria Smith
Department of Biochemistry, Stanford University, CA
12:30 - 1:30 pm Lunch
In Search of Unknown Mutations
1:30 - 2:00 pm Hybrid SSCP methods for detecting essentially 100% of
mutations
Hiroshi Nishino
Department of Biochemistry and Molecular Biology,
Mayo Medical School, Rochester, MN.
2:00 - 2:30 pm Enzymatic methods for mutation detection.
Christopher D. Earl
Avitech Diagnostics, Malvern, PA
2:30 - 3:00 pm Development and application of an in vivo method to
detect DNA sequence variation.
Malek Faham
Department of Genetics, Stanford University, CA
3:00 - 3:30 pm High-performance liquid chromatography of nucleic acids
and its application to comparative DNA sequencing.
Peter Oefner
Department of Biochemistry, Stanford University, CA
3:30 - 4:00 pm Coffee Break
