IUBio

needing an advice

idl at AGERI.SCI.EG idl at AGERI.SCI.EG
Mon Dec 2 14:45:37 EST 1996


I'm working at the National Plant Genetic Resources Unit/ Egypt. I have 
started my M.Sc.program from 3 months ago about the characterization and 
evaluation of some cucurbits growing under Egyptian conditions, using 
morphological characters in addition to protein electrophoresis and isozyme 
analyses. I have a big problem concerning the seeds collected by some people 
two years ago whom don't know any thing about international rules of 
collection which we call collection form or passport data.
By the way, the number of cucurbit collections are 45, 39 are melon and 6 
are snake cucumber, collected from all over the country from farmers which 
means that this collections couldn't be pure lines. Now, I want to know is 
it right to apply the protein electrophoresis and isozyme analysis on these 
seeds directly or I have to replant the seeds of each collection and then 
choose 5 fruits from different plants randomly and apply the previous 
techniques on their seeds. I hope every body get my message and thank you in 
advance for your cooperation.
 
 
Abdel Mohsen Tohamy
Agricultural Genetic Engineering Research Institute
Agricultural Research Center
9 Gamaa Street
Giza 12619
Egypt
Phone# 202-572-7831 
Fax # 202- 568-9519




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