I've recently transferred from academia into industry and have stumbled
onto a problem.
I need a tag that can be used N-terminally to quantify expression of its
fusion partner - ideally in vivo. I was thinking about GFP, or one of
its derivates, but I think this protein might be patented. I've tried
searching for relevant patents without success. Can anyone confirm that
I need a license for the use of GFP (or any of its derivates) for
industrial gene expression quantification?
What alternatives are there? Can luciferase be used in vivo (will
luciferin enter the cells)? Is its use protected?
I'd rather not use antibodies since this is expresive, cumbersome and
must be done in vitro.
Trond Erik Vee Aune