Dear Joachim Hogg,
We have expressed a membrane transporter, P-glycoprotein as a fusion
protein to GFP. The expression of Pgp was quantified (both in fluorescence
microscopy and FACS) and the activity was quantified either by its effect
on fluorescent substrates or by the activity of its substrates. The
results are in the following article:
Y. Chen and S. M. Simon. In situ biochemical demonstration that
P-glycoprotein is a drug efflux pump with broad specificity. J.Cell Biol.
148 (5):863-870, 2000.
- Sandy Simon
>X-Authentication-Warning: niobium.hgmp.mrc.ac.uk: news set sender to
><news> using -f
>From: joachogg at biocip.uni-freiburg.de (Joachim Hogg)
>Subject: GFP Fusion Proteins
>Date: 6 Nov 2000 12:43:47 -0000
>Organization: BIOSCI/MRC Human Genome Mapping Project Resource Centre
>X-To: fluorpro at hgmp.mrc.ac.uk>To: fluorpro at hgmp.mrc.ac.uk>>Could anyone direct me to a reference for Fusions between GFP and Enzymes
>(the enzyme has to keep its activity).
>Any suggestions appreciated
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