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catecholamine fluorescence and pH

Beat Ludin bludin at lis.ch
Wed May 31 16:52:24 EST 2000

Sorry, I can't answer your questions. I'm just curious, have you 
checked whether the fluorescence returns (immediately) upon 
neutralization? The would suggest a simple de-protonation.



>Hello - I am not quite sure, this is the right newsgroup to ask this
>question in - but I'm more or less desperate.....
>I would like to know how the fluorescence spectra of  the catecholamines
>adrenaline/ephinephrine and noradrenaline/norepinephrine) change with
>pH .
>The compounds seem to decompose in even slightly basic media (e.g.
>pH=8.5) - the fluorescence vanishes. Is this due to oxidation of
>phenoles??? – or is it another cause?  Will you under any circumstances
>be able to measure catecholamine fluorescence in basic solution?
>Furthermore, does anybody know where the catecholamine's chemical
>and fluorescence properties might be described in detail?
>Thanks in advance

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