Does anybody have been successfull in overexpressing DsRed in E. coli ?
If yes, what were the expression conditions ? (how many time induction,
temperature of induction, E coli strain....).
I have also problems for cloning the DsRed coding sequence (from the
clontech plasmid pDsRed-N1).
After cloning in my vector, the restriction map is OK but there is no
fluorescence (Microscope or spectrofluorimeter checking).
Any help is welcome.
Thank you very much
Dr. Isabelle Sagot
AG Hurt, 4. OG
Im Neuenheimer Feld 328
Email: hn7 at ix.urz.uni-heidelberg.de