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fixation of GFP

Derya is an idiot unutmaz at MCBI-34.MED.NYU.EDU
Wed Feb 24 13:17:22 EST 1999

 I generated GFP knockin mice. I found GFP fluorescence disappears after
freeze-thaw, unless pretreated with 4% paraformaldehyde. However, such
fixation will destroy/mask the cell surface epitope. Therefore, I can
not do staining for cell surface markers.

Does anyone have any experience on this?


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