Last year, Roger Tsien has described a new approach to a fluorescent
vital dye for specific proteins. It involves tagging the protein with a
small tag which then serves as a target for a fluorescent pro-ligand:
4',5'-bis(1,3, 2- dithioarsolan-2-yl)fluorescein
Griffin BA, Adams SR & Tsien RY. (1998). Specific covalent labeling of
recombinant protein molecules inside live cells. Science 281, 269-272.
Has anybody had luck with this new approach?