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neoGFP fusion

Louise Neville louise.neville at CHMEDS.AC.NZ
Tue Feb 16 20:49:00 EST 1999


HI all

has anyone had experience with the neoGFP fusion vector from Clontech??  I
have transfected it with FuGENE into cos-7 cells and found it is
significantly less efficient than straight EGFP to the extent that it is
difficult to locate any transfected cells and the signal is very dim.
I would appreciate any advice, there is no mention of this variation in
expression in the handbook or catalogue

thanks

Louise
Louise Neville				Phone (+64)(3)364-0880
Gene Structure and Function Lab		Fax   (+64)(3)364-0750
Cytogenetics Unit
Christchurch School of Medicine		email louise.neville at chmeds.ac.nz
Christchurch, New Zealand



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