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Your questions regarding GFP-promotorassays.

Guido Mq ttu0j0 at SP2.POWER.UNI-ESSEN.DE
Tue Feb 2 15:05:12 EST 1999


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Hello,=20
with great inerest I read your posting in bio.net.
 I'm now at the point, you have been in july98: I want to construct a =
reporter to measure the downregulation of a promotor. I think the best =
way would be to combine two GFP-variants in one plasmid: BFP under =
contol of CMV and my poromotor controling EGFP. This would enable me to =
measure the GFP/BFP-ratio and maybe detecting minor changes for my =
promotor. Did you recieve any interesting replies to your question, or =
did you come any further in your attempt?
Any suggestions and help would be fine !

Guido Marquitan (PhD-Student)
Innere Klinik/Tumorforschung
Lab. Schuette/Opalka
Hufelandstr.55
D-45122 Essen
GERMANY
Tel. +49-201-723-2017
guido.marquitan at uni-essen.de

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<DIV><FONT face=3DArial size=3D2>Hello, </FONT></DIV>
<DIV><FONT face=3DArial size=3D2>with great inerest I read your posting =
in=20
bio.net.</FONT></DIV>
<DIV><FONT face=3DArial size=3D2>&nbsp;I'm now at the point, you have =
been in=20
july98: I want to construct a reporter to measure the downregulation of =
a=20
promotor. I think the best way would be to combine two GFP-variants in =
one=20
plasmid: BFP under contol of CMV and my poromotor controling EGFP. This =
would=20
enable me to measure the GFP/BFP-ratio and maybe detecting minor changes =
for my=20
promotor. Did you recieve any interesting replies to your question, or =
did you=20
come any further in your attempt?</FONT></DIV>
<DIV><FONT face=3DArial size=3D2>Any suggestions and help would be fine=20
!</FONT></DIV>
<DIV>&nbsp;</DIV>
<DIV><FONT face=3DArial size=3D2>Guido Marquitan =
(PhD-Student)</FONT></DIV>
<DIV><FONT face=3DArial size=3D2>Innere =
Klinik/Tumorforschung</FONT></DIV>
<DIV><FONT face=3DArial size=3D2>Lab. Schuette/Opalka</FONT></DIV>
<DIV><FONT face=3DArial size=3D2>Hufelandstr.55</FONT></DIV>
<DIV><FONT face=3DArial size=3D2>D-45122 Essen</FONT></DIV>
<DIV><FONT face=3DArial size=3D2>GERMANY</FONT></DIV>
<DIV><FONT face=3DArial size=3D2>Tel. +49-201-723-2017</FONT></DIV>
<DIV><FONT face=3DArial size=3D2><A=20
href=3D"mailto:guido.marquitan at uni-essen.de">guido.marquitan at uni-essen.de=
</A></FONT></DIV></BODY></HTML>

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