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Two GFP variants in one vector- is it possible?

Guido Mq ttu0j0 at SP2.POWER.UNI-ESSEN.DE
Tue Feb 2 14:13:43 EST 1999


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I want to construct a reporter-vector for the inactivation of a =
promotor. For this purpose it would be verry helpful, if I could place =
two variants of GFP in the same plasmid (BFP constitutively expressed by =
e.G. CMV-promotor and EGFP controlled by the promotor I want to look =
at). This would enable me to measure the ratio of BFP to GFP and so even =
small changes could be measured. Did anyone try to do so as well, and =
are there any problems  (for example rearrangements between the two very =
similar sequences)?

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<DIV><FONT color=3D#000000 face=3DArial size=3D2>I want to construct a =
reporter-vector=20
for the inactivation of a promotor. For this purpose it would be verry =
helpful,=20
if I could place two variants of GFP in the same plasmid (BFP =
constitutively=20
expressed by e.G. CMV-promotor and EGFP controlled by the promotor I =
want to=20
look at). This would enable me to measure the ratio of BFP to GFP and so =
even=20
small changes could be measured. Did anyone try to do so as well, and =
are there=20
any problems&nbsp; (for example rearrangements between the two very =
similar=20
sequences)?</FONT></DIV></BODY></HTML>

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