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pEGFP-N1?

Paul Kitts kitts at NCBI.NLM.NIH.GOV
Thu Apr 29 11:42:33 EST 1999


> Has anyone gotten away with using ampicillin as a
> selective antibiotic for this vector

I'll be amazed if they have!
pEGFP-N1 does not have an ampicillin resistance gene, therefore, you will have 
to use kanamycin.
-=Paul=-

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"  Paul Kitts, Ph.D.               NIH/NLM/NCBI                "
"  Email: kitts at ncbi.nlm.nih.gov   Bldg 38A, 8N803             "
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To: fluorpro at net.bio.net
From: Dr Lester Davids <lester at uctgsh1.uct.ac.za>
Subject: pEGFP-N1?
Date: Thu, 29 Apr 1999 17:16:39 +0200

I am using the pEGFP-N1 vector and really struggling to transform high
competent JM109's. Has anyone gotten away with using ampicillin as a
selective antibiotic for this vector or must one use kanamycin?

Also, has anyone had success with using more than 1-10ng DNA of this
vector in the transformation reaction?

Thanx





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