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pEGFP-N1?

Dr Lester Davids lester at uctgsh1.uct.ac.za
Thu Apr 29 10:16:39 EST 1999


I am using the pEGFP-N1 vector and really struggling to transform high
competent JM109's. Has anyone gotten away with using ampicillin as a
selective antibiotic for this vector or must one use kanamycin?

Also, has anyone had success with using more than 1-10ng DNA of this
vector in the transformation reaction?

Thanx



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