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VersaFlow

Dr. Robert Gay r.gay at unsw.edu.au
Mon Apr 19 21:21:27 EST 1999


Dear FPers

Has anyone had any experience using the BioRad VersaFlour Fluorometer for
quantitation of GFP and/or EGFP ?

Is it any good ?

It appears that you have to set up a standard curve using recombinant
protein to aid the quatitation.  Is a GFP standard curve required for GFP
quantitation and a EGFP standard curve for EGFP quantitation or is there
some overlap ?

Hope someone can help.

Keep exciting and emitting

Robert
Dr Robert Gay

Post Doctoral Research Fellow
CRC for Biopharmaceutical Research
Department of Biotechnology
University of New South Wales
Sydney, NSW 2052, Australia

Tel : +61(0)2-9385-1892
Fax : +61(0)2-9313-6710




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