IUBio Biosequences .. Software .. Molbio soft .. Network News .. FTP

Cameleons and EYFP intensity

Trent Watkins trent at KOSH5.BERKELEY.EDU
Wed Apr 14 16:32:35 EST 1999


You might try looking at a recently published paper that addresses some of
these issues:

Miyawaki A, et al.  Dynamic and quantitative Ca2+ measurements using
improved cameleons.  Proc. Natl. Acad. Sci. USA.  96: 2135-2140 (1999).

Trent Watkins
Research Associate / Koshland Lab
University of California at Berkeley


On 14 Apr 1999, EMMA STAVROPOULOS wrote:

> 
> I'm expressing a conjugate containing ECFP and EYFP from  
> 'yellowcam-2' ( Miyawaki et al.,(1997)),  and I have found that 
> the EYFP fluorescence intensity is considerably lower than reported 
> in the paper.  This may be due to my particular construct, which 
> contains a different binding region to the original.  However, I have 
> heard reports that EYFP intensity in original constructs  varies 
> with pH and  ion concentration.  Can anybody support this?  If so, 
> what conditions gain optimal EYFP intensity?
> 
> I would greatly appreciate any advice or comment from anyone using 
> these 'Cameleons', in ANY system.
> 
> Thank-you,
> 
> Emma Stavropoulos.
> 
> 
> e:mail:  Emma.Stavropoulos at ed.ac.uk
> 
> Emma Stavropoulos
> Institute of Cell and Molecular Biology
> University of Edinburgh
> 
> Tel (+44) (0)131 6505319
> Fax (+44) (0)131 650 5392
> 
> 
> 
> 
> 
> 
> 
> Emma Stavropoulos
> Institute of Cell and Molecular Biology
> University of Edinburgh
> 
> Tel 0131 6505319
> 
> 




More information about the Fluorpro mailing list

Send comments to us at biosci-help [At] net.bio.net