> Does anyone know how to overlay 2 images of the same field using Adobe
> Photoshop 4.0? I am Dapi staining and also using a membrane counterstain
> and would like to see the relative location of the nucleoid in the
> cells. Phase microscopy has given me poor results due to the size of the
> cells. Any help would be greatly appreciated.
>> Jarrat Jordan
> University of Georgia Microbiology
>jarrat at arches.uga.edu
Two things: I think it is much easier to overlay a DIC image than a phase
contrast image; the phase image is often too dark to be able to see the
Secondly, can use "merge as a composite" and not "as an average" in
photoshop? I find that this method works best (however, I am using
Openlab to do the merge first, and photoshop to clean it up if necessary).