On Fri, 2 Oct 1998 roscoe01 at globalnet.co.uk wrote:
> Does anyone know where I can get more information on this topic?
I recently started examining the same question. I'll suggest to you the
best of what I've found so far. Try this reference for a chart containing
many examples of applications of GFP mutants and their references:
Cubitt AB, et al. Trends in Biochemical Sciences. 1995, 20(11): 448-55.
CLONTECH provides vectors containing GFP and BFP which have been optimized
for human codon usage (EGFP and EBFP) -- I'm sure they would have
lists of applications which have used their products.
Other very recent reviews might help you as well:
Tsien RY, Miyawaki A. Science, 1998 Jun 19, 280(5371): 1954-5.
Tsien RY. Annual Review of Biochemistry, 1998, 67: 509-44.
Any other suggestions or advice on where to get info on optimizing and
using GFP and BFP would be appreciated.