A friend of mine experiences the following problem:
it appears as if samples double labelled with phycoerythrin
and FITC "loose" FITC label.
When she does single labellings, they work fine. However,
in double labelled samples only the PE label is visible and
the FITC label is gone.
I have no experience with PE whatsoever, so I am clueless.
Thanks a lot
Dr. Michal Opas
Department of Anatomy & Cell Biology
University of Toronto
1 King's College Circle
Medical Sciences Building
Toronto, Ontario, M5S 1A8 Canada
phone: (416) 978-8947
fax: (416) 978-3954
e-mail: m.opas at utoronto.ca
www homepage: http://www.utoronto.ca/anatomy/opas/start.htm