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Anti-Fade for GFP

Wolfgang Schechinger wgschech at med.uni-tuebingen.de
Fri Mar 27 17:32:41 EST 1998


<snip snip>
Science 217, 1252-5 (1982) says 5% n-Propyl gallate in glycerol does 
the job. Of course it then was not tested on GFP.
To me, it seems to preserve S65T fluorescence quite well.

However, I recently tried Permafluor embedding 
medium from Immunotech (Marseille, France) with initially very good
results. But when I examined the slides two weeks later, I observed a 
high bacjground fluorescence with FITC filters. H33258 (bisbenzimide) 
in contrary was looking quite well. 
Was it wrong to store the slides at RT? Would they better be kept in 
the fridge?

Wolfgang

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Wolfgang Schechinger         
University of Tuebingen
email: wgschech at med.uni-tuebingen.de
http://www.medizin.uni-tuebingen.de/~wgschech/research.htm
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