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GFP Flourometric Assay

Ricardo Hermosilla hermosilla at fmp-berlin.de
Fri Mar 27 05:22:43 EST 1998


Hi GFP people

Does any one have a good protocol for GFP quantification in a
fluorometer. We are actually working
with a membrane protein tagged with GFP in MDCK cells. Our problem is to
get the tagged protein into solution without quenching GFP fluorescence.
We have already tried whole and sonicated cells without success.

Thank you for all replies

Ricardo Hermosilla
FMP Berlin





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