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Is GFP expression homogenous in monoclonal cell population?

Yuan Shen jh7 at ACSU.BUFFALO.EDU
Fri Mar 13 09:33:36 EST 1998


Hi,

Did anyone notice heterogenous expression of EGFP in monoclonal cell
population? 

We observed that even monoclonal NIH 3T3 cells stably transfected with
pEGFP-C1 showed heterogenous expression of EGFP. This led to less
percentage of postive(green) cells  in a population on FACS. 

Does anyone know if this is usual? Does anyone know how they could achieve
near 2 log level increase of fluorescence intensity in a population?(Shown
by the right shift of transfected cells compare to wild-type in FACS
histogram in published papers). Is this achieved by constantly sorting the 
cells?

Thanks in advance for any suggestion.




***************************************
Yuan Shen
Dept. of Microbiology/Immunology
Roswell Park Cancer Institute
State University of New York at Buffalo





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