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urgent (sorry) : Fixing GFP for phalloidin stainining of cytoske

Wolfgang Schechinger wgschech at med.uni-tuebingen.de
Wed Mar 11 06:49:33 EST 1998


Hi all. 

Since acetone will kill GFP fluorescence: How will I get the 
cytoskeleton of 293 fibroblasts and smooth muscle cells stained? 
Rhodamine-phalloidin and coumarin-phalloidin work well on acetone 
fixed cells, but in methanol - nope. How might I get out of this 
dilemma? Would adding the phalloidin dye to the culture medium help, 
depite it will hurt cells?
Could I use another fixing procedure, actin dye or what should I do?

Standing by for your suggestions!

Wolfgang

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Wolfgang Schechinger         
University of Tuebingen
email: wgschech at med.uni-tuebingen.de
http://www.medizin.uni-tuebingen.de/~wgschech/research.htm
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