Since acetone will kill GFP fluorescence: How will I get the
cytoskeleton of 293 fibroblasts and smooth muscle cells stained?
Rhodamine-phalloidin and coumarin-phalloidin work well on acetone
fixed cells, but in methanol - nope. How might I get out of this
dilemma? Would adding the phalloidin dye to the culture medium help,
depite it will hurt cells?
Could I use another fixing procedure, actin dye or what should I do?
Standing by for your suggestions!
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University of Tuebingen
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