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Fixing GFP: Glutaraldehyde better that Formaldehyde?

Julie R. Hens jrhens at WAM.UMD.EDU
Sun Mar 8 04:42:15 EST 1998


Wolfgang and all,
I recently transfected HC11 cells with EGFP. I fixed the cells with 2%
Paraformaldehyde. I didnt see any loss of fluorescence b/w fixed and not
fixed cells. Hope this helps? 
Julie 

On 7 Mar 1998, Wolfgang Schechinger wrote:

> Hi all, 
> 
> Wonder if it's better to fix cells with glutaraldehyde 
> (crosslinking soluble GFP) that formaldehyde.
> In the last discussion, many have said that formaldehyde would be 
> quite suitable, also the clontech manual says that, but someone noted 
> that soluble GFP (proteins not attached firmly to others?) would 
> disappear during fixation.
> Is glutaraldehyde likely to fix that problem or will it 
> slef-crosslink GFP and abolish the fluorescence?
> Anyone who has tried this out yet?
> 
> Wolfgang
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> Wolfgang Schechinger         
> University of Tuebingen
> email: wgschech at med.uni-tuebingen.de
> http://www.medizin.uni-tuebingen.de/~wgschech/research.htm
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