Wolfgang and all,
I recently transfected HC11 cells with EGFP. I fixed the cells with 2%
Paraformaldehyde. I didnt see any loss of fluorescence b/w fixed and not
fixed cells. Hope this helps?
On 7 Mar 1998, Wolfgang Schechinger wrote:
> Hi all,
>> Wonder if it's better to fix cells with glutaraldehyde
> (crosslinking soluble GFP) that formaldehyde.
> In the last discussion, many have said that formaldehyde would be
> quite suitable, also the clontech manual says that, but someone noted
> that soluble GFP (proteins not attached firmly to others?) would
> disappear during fixation.
> Is glutaraldehyde likely to fix that problem or will it
> slef-crosslink GFP and abolish the fluorescence?
> Anyone who has tried this out yet?
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> Wolfgang Schechinger
> University of Tuebingen
> email: wgschech at med.uni-tuebingen.de>http://www.medizin.uni-tuebingen.de/~wgschech/research.htm> -------
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