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Fixing GFP: Glutaraldehyde better that Formaldehyde?

Wolfgang Schechinger wgschech at med.uni-tuebingen.de
Sat Mar 7 20:36:27 EST 1998


Hi all, 

Wonder if it's better to fix cells with glutaraldehyde 
(crosslinking soluble GFP) that formaldehyde.
In the last discussion, many have said that formaldehyde would be 
quite suitable, also the clontech manual says that, but someone noted 
that soluble GFP (proteins not attached firmly to others?) would 
disappear during fixation.
Is glutaraldehyde likely to fix that problem or will it 
slef-crosslink GFP and abolish the fluorescence?
Anyone who has tried this out yet?

Wolfgang
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Wolfgang Schechinger         
University of Tuebingen
email: wgschech at med.uni-tuebingen.de
http://www.medizin.uni-tuebingen.de/~wgschech/research.htm
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