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eGFP expression in e.coli

Chris Marquis C.Marquis at unsw.edu.au
Wed Jan 29 21:42:38 EST 1997


dear all,

has anyone tried to express any of the GFP mutants (S65T, EGFP, RSGFP) in 
e.coli? most of the commercial vectors seem to have codons optimised for 
mammalian cell expression.

i would appreciate any feed back on this. thanks in advance

chris marquis





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