As I guessed there is a high interest in the issue of using a slide
projector (or something similiar simple) for the excitation of GFP. Since I
have been asked by several interested colleques, whether I have received
answer on my questions concerning excitation with a slide projector, I post
this answer to the newsgroup:
>>Dear Christoph Reichel;
>>I've used a slide projector with a blue glass filter. In general, any
>filter that cuts off before 450nm should work. Just rig is to be the same
>size as a slide.
>>I've found that a blacklight (bought at Spencer's in the mall) is far
>superior to a slide projector for one major reason. Slide projectors use
>incandescent bulbs. These bulbs don't emit much blue light and almost no
>UV. Fluorescent lighting is not limited in this respect. These bulbs are
>pre-filtered and work quite well for bacterial colonies.
>>Seth Jon Davis sjdavis1 at students.wisc.edu>UW-Madison Horticulture Lab (608) 262-1622
>Madison, Wisconsin 53703 FAX (608) 262-4743
Any further suggestions will be highly appreciated,
The Scripps Research Institute
Dept. Cell Biology, BCC 206
10550 North Torrey Pines Rd.
La Jolla, CA 92037
phone: (619) 784-2918
fax: (619) 784-2994