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Quantification of GFP and Expression at 33C

Lapointe, Renee lapointr at POST.QUEENSU.CA
Tue Jan 7 15:56:50 EST 1997


Hi,
I am interested in the use of GFP for quantification of baculovirus
promoter expression in a transient assay. What method of quantification of
GFP could I use, and would it be possible to detect and quantify two
different GFP variants under different promoters in the same cells. Could
anyone refer me to specific references on quantification assays.

Another concern that I have is the efficiency of the GFP at 33C. Does
anyone  have experience on this?

Thank you for your help

Renee Lapointe
Department of Microbiology and Immunology
Queen's university





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