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Clontech GFP

Chris Lau clau at ITSA.UCSF.EDU
Wed Feb 26 16:56:55 EST 1997


Yes.  It works better in our hands.

_______________________________________________________________________


On Wed, 26 Feb 1997, Alex Harvey wrote:

> Is anyone using Clontech's pEGFP reporter vectors?  I want to compare
> expression of the F64L/S65T double mutant to the same with "humanized"
> codons.  Clontech claims the change in codon usage results in around a
> 7-fold increase in fluorescent signal (in CHO cells) - can anyone verify
> this (in any cell type)?  I'm using GFP as a reporter in chicken
> blastodermal cells.
> 
> Thanks for any input.
> 
> Gord Speksnijder
> Department of Genetics
> B410 Life Sciences
> University of Georgia
> Athens, GA 30602-7229
> phone (706) 542-1420
> fax   (706) 542-1413
> email  gord at bscr.uga.edu
> 
> 

_____________________________________________________________________________

Chris Lau, Ph.D. 				Tel: 415 476-8839 
Division of Cell and Developmental Genetics 	FAX: 415 502-1613 
Department of Medicine, VAMC-111C5 
University of California, San Francisco 	E-mail : clau at itsa.ucsf.edu 
4150 Clement Street
San Francisco, CA 94121






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