Probably something of a trivial question, but is there any discrepancy
in the estimated size of wild type (or even S65T) GFP? It's just that most
papers cite it's *estimated* size at around 28kD, but in the Ormo paper on
GFP structure, mass spec. data gave a figure around 31kD. For what it's
worth, on our SDS-PAGE gels it runs right with the 31kD marker, but I'm
not vouching for the accuracy of that at all!
What is the generally held view nowadays?
Thanks for any responses,
CRC Chromosome Molecular Biology Group
Department of Biochemistry
University of Oxford
Tel: +44 (0)1865 275222
Fax: +44 (0)1865 275283