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general gfp questions

A. Kania " ak316" at columbia.edu
Mon Feb 17 20:50:36 EST 1997


Dear GFP-ers, 

I would like to find out a few things about gfp, or the new modified
versions of gfp such as the ones sold by Clonetech. 
I plan to express a gfp fusion protein where a nuclear protein is fused
to the Nterm of gfp. I plan to express this in the mouse and FACS sort
live cells obtained from such mice.

1. does anyone have any experience with FACS sorting of gfp expressing
cells ? Does it work with mesenchymal embryonic cells ?

2. is there a convenient source of gfp DNA that would have the following
structure:
	multiple cloning site (MCS)-gfp- poly A addition site - MCS  

3. does this group have a FAQ ? If so, where could i find it ?

			Thanks for your help, 

						Artur Kania



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