I am interested in using two different colored forms of GFP as reporters
to compare mutations in a promoter with a "normalization" promoter
signal on a cell-by-cell basis. What advice or guidance would
experienced users suggest regarding selection of reporters (including
vendors), choice of color ranges and compatibility with commonly
available fluorescence microscopy filters.
M.P. Frosch, MD, PhD
Neuropathology | Center for Neurologic Diseases
Brigham & Women's Hosp. | Brigham & Women's Hosp.
75 Francis Street | 77 Avenue Louis Pasteur (HIM 764)
Boston, MA 02115 | Boston, MA 02115
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617 732-7532 (secretary) | 617 525-5240 (lab/office)
617 975-0944 (fax) | 617 525-5305 (fax)
mpfrosch at bics.bwh.harvard.edu | frosch at dsg.harvard.edu