Date: Wed, 22 May 96 09:26:39 +0100
From: christian a geltinger <geltinger at gsf.de>
Subject: Re: GFP-transgenic mammalian cells?
To: dietzel at sun0.urz.uni-heidelberg.de (Steffen Dietzel)
X-Mailer: LeeMail 2.0.4
Message-Id: <ADC88E50 at haema106.gsf.de.>
>>I have heard and read already various reports about successful
>*transfection* of GFP-fusion proteins DNA into mammalian cells, but I can
>not remember any stable *transgenic* mammalian gfp-producing cells.
>Have I just not found the respective reports? If there are indeed none, is
>there any idea why it does not work?
it works ! At least in Burkitt+s lymphoma cell lines, which are of B-cell
origin. I+ve established cell lines stably transfected with a high- and
low-expressing gfp-construct 6 weeks ago, they grew out quite esily (nice to
follow them growing on a 96 well plate by the way) and are still living happily
with almost no change in brightness of gfp-fluorescence. So at least these cells
have found a way to deal with gfp-turnover and degradation (some of the cells
actually didnt so they turned into big green blastoid-like cells). This work has
not been published yet, so you surely dont find it the literature.
Hope this helps !
C h r i s t i a n A. G e l t i n g e r
Institut fuer Klinische Molekularbiologie und Tumorgenetik
GSF-Forschungszentrum fuer Umwelt und Gesundheit
Phone: xx49/89/7099-509 Marchioninistr. 25
FAX: xx49/89/7099-500 D-81377 Muenchen
e-mail: geltinger at gsf.de Ger