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GFP-transgenic mammalian cells?

christian a geltinger geltinger at gsf.de
Wed May 22 02:25:28 EST 1996


Date: Wed, 22 May 96 09:26:39 +0100
From: christian a geltinger <geltinger at gsf.de>
Subject: Re: GFP-transgenic mammalian cells?
To: dietzel at sun0.urz.uni-heidelberg.de (Steffen Dietzel)
X-Mailer: LeeMail 2.0.4
Message-Id: <ADC88E50 at haema106.gsf.de.>

>Hello everybody,
>
>I have heard and read already various reports about successful
>*transfection* of GFP-fusion proteins DNA into mammalian cells, but I can
>not remember any stable *transgenic* mammalian gfp-producing cells.
>Have I just not found the respective reports? If there are indeed none, is
>there any idea why it does not work?
>
>Steffen Dietzel
>


Dear Steffen, 
it works ! At least in Burkitt+s lymphoma cell lines, which are of B-cell 
origin. I+ve established cell lines stably transfected with a high- and 
low-expressing gfp-construct 6 weeks ago, they grew out quite esily (nice to 
follow them growing on a 96 well plate by the way) and are still living happily 
with almost no change in brightness of gfp-fluorescence. So at least these cells 
have found a way to deal with gfp-turnover and degradation (some of the cells 
actually didnt so they turned into big green blastoid-like cells). This work has 
not been published yet, so you surely dont find it the literature.

Hope this helps !

C h r i s t i a n  A. G e l t i n g e r

Institut fuer Klinische Molekularbiologie und Tumorgenetik
GSF-Forschungszentrum fuer Umwelt und Gesundheit

Phone:  xx49/89/7099-509                    Marchioninistr. 25 
FAX:    xx49/89/7099-500                      D-81377 Muenchen 
e-mail: geltinger at gsf.de                               Ger




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