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Immunofluorescence using anti-rGFP Antiserum

Jamie White jwhite at embl-heidelberg.de
Tue May 21 10:23:04 EST 1996


In article <31A08A0F.3B52 at lclark.edu>, lochner at LCLARK.EDU (Janis Lochner) wrote:

> Has anyone tried using the Clontech anti-rGFP 
> antiserum in an immunofluorescence study to confirm 
> GFP expression ?

Yes.  In HeLa cells. See Figure 1 in

http://www.embl-heidelberg.de/~jwhite/project/prelim/preliminary.html

People here (EMBL) have also used it in MDCK cells.

jamie

-- 
Jamie White
Light Microscopy Group
Cell Biophysics Programme
European Molecular Biology Laboratory
Meyerhofstrasse 1, Postfach 10.2209
D-69117 Heidelberg Germany
+49 6221 / 387 367 voice
+49 6221 / 387 242 fax 
finger jwhite at phenix.embl-heidelberg.de



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