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fluorescein bleaching

Yasushi OKADA yokada at kinesin.kaibo1.m.u-tokyo.ac.jp
Mon May 20 09:48:05 EST 1996


In article <319D2C08.4E28 at clontech.com> Paul Kitts
<pakitts at clontech.com> writes:

> I hear that fluorescein is quite sensitive to photobleaching. 
> Intense light may bleach the dye without harming the protein.

Free radicals formed during photobleaching sometimes inactivate the
protein.  

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 * Yasushi Okada, MD.           | Email:yokada at m.u-tokyo.ac.jp   *
 * Dept. Anatomy & Cell Biology | Tel:81-3-3812-2111 ext.3336    *
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