John McDonald wrote:
>> I would like to know if there is a procedure out there to chemically
> bleach a fluorescent probe that is attached to a protein while
> maintaining the integrity of that protein. The probe is fluorescein
> isothiocyanate. My initial attmpts at oxidation and acylation failed
> to bleach the fluorescence. Has anyone tried to do this or have an
> idea that might work? Thanks a lot.
I hear that fluorescein is quite sensitive to photobleaching.
Intense light may bleach the dye without harming the protein.
-= Paul =-
! Paul Kitts !
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