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fluorescein bleaching

Paul Kitts pakitts at clontech.com
Fri May 17 20:46:48 EST 1996


John McDonald wrote:
> 
> I would like to know if there is a procedure out there to chemically
> bleach a fluorescent probe that is attached to a protein while
> maintaining the integrity of that protein. The probe is fluorescein
> isothiocyanate. My initial attmpts at oxidation and acylation failed
> to bleach the fluorescence. Has anyone tried to do this or have an
> idea that might work? Thanks a lot.
> 

I hear that fluorescein is quite sensitive to photobleaching. 
Intense light may bleach the dye without harming the protein.

-= Paul =- 

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! Paul Kitts                                                !
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