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GFP that cannot dimerize?

Markus Maniak maniak at dicty.biochem.mpg.de
Thu May 16 03:56:10 EST 1996


Dear GFP researchers,

I have experienced a problem when expressing a GFP-fusion which is
targetted to a vesicle membrane. These vesicles unexpectedly agglutinate
inside the cells, and I wonder whether they stick together by the inherent
dimerization property of GFP (also discussed by Roger Tsien about  six
months ago in this newsgroup).
Does anybody know of a GFP mutant which is unable to dimerize?
Thanks for your help!

Markus Maniak (maniak at vms.biochem.mpg.de)



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