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fluorescein bleaching

John McDonald jfmcdon at BIOSCI.CBS.UMN.EDU
Tue May 14 11:56:39 EST 1996


I would like to know if there is a procedure out there to chemically 
bleach a fluorescent probe that is attached to a protein while 
maintaining the integrity of that protein. The probe is fluorescein 
isothiocyanate. My initial attmpts at oxidation and acylation failed 
to bleach the fluorescence. Has anyone tried to do this or have an 
idea that might work? Thanks a lot.

																																			John McDonald
																																			jfmcdon at biosci.cbs.umn.edu



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