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Texas red vs FITC

Jennifer_Kramer_at_notes at SMTP.CSPI.COM Jennifer_Kramer_at_notes at SMTP.CSPI.COM
Sun May 12 08:34:33 EST 1996


  It is true that fluorescein photobleaches more rapidly than Texas Red.
  However, the method of visualization of your probes is also important.
  If you have a conventional widefield microscope equipped with an arc lamp
  and you are viewing the probes through the eyepieces of the microscope,
  some people find fluorescein to appear brighter, as our eyes are more
  sensitive to green than red.  If you are using a CCD camera or
  photographic film to visualize your probes, however, Texas Red might
  appear brighter because CCD cameras and film have better spectral
  response to the red than the green.  If you are using a confocal
  microscope equipped with an argon ion or krypton/argon ion laser,
  fluorescein will probably be the brighter probe, because it is more
  efficiently excited by the laser than Texas Red, and the photomultiplier
  tubes used to detect the emission signal are more sensitive to green than
  red.

  Jennifer Kramer
  Applications Scientist
  Scanalytics, Inc.

  To:       fluorpro @ net.bio.net at smtp at CCMAIL
  cc:        (bcc: Jennifer Kramer)
  From:     ming @ msvax.mssm.edu at smtp at CCMAIL
  Date:     05/11/96 04:07:00 PM
  Subject:  Re: Texas red vs FITC


In article <318FC394.7915 at sct.gu.edu.au>, "H. Woo" <h.woo at sct.gu.edu.au>
writes: >Has anyone observed that the red fluorescence from texas
red-labelled >probes is easier to see than the green fluorescence from
FITC-labelled >probes in whole cell hybridization?

FITC decays faster than others. If you do not use double labelling, cys3
from Amersham is the best.




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