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gfp in mammalian cells

Brenda Schulman Schulman at HELIX.MGH.HARVARD.EDU
Thu May 9 12:12:36 EST 1996


I would like to use gfp as a vital marker for gene expression in mammalian
cells.  Is it possible to see gfp @ 37C?  Are there new variants that
fold/autooxidize faster @ 37?  How long is the delay between expression and
fluorescence in mammalian cells, and which variant is the fastest for this
purpose?

THANK YOU VERY MUCH!!

Brenda Schulman
schulman at helix.mgh.harvard.edu





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