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Fluorescent plate reader

milstone milstone at rascal.med.harvard.edu
Thu May 9 08:14:57 EST 1996


Karen P Scott <kps at rri.sari.ac.uk> wrote:
>>The following is a reply Bob MacDonald sent me concerning the use of a
>fluorescent plate reader.
>
>>It will take a plate reader of unusual sensitivity if it works at all. 
>>...If one were to, say, remove  the cells from a 6 cm dish and put 
>>them all in one well of a 96 well plate, it might work.

I believe that some (probably more expensive) plate readers will 
concentrate particulate samples, such as cells, in 96 well plates by 
filtration and read the fluorescent signals from the resulting smaller 
areas. This is an automated procedure and is claimed to enhance 
sensitivity significantly. This might facilitate the type of 
concentration that you suggest without the need for additional manual 
sample processing.





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