IUBio Biosequences .. Software .. Molbio soft .. Network News .. FTP

GFP-detection limits

Paul Kitts pakitts at clontech.com
Fri May 3 19:18:41 EST 1996


>> Does anyone know what the lower limits for detection of GFP in 
>> mammalian expression systems is? 
> 
> Niswender KD, Blackman SM, Rohde L, Magnuson MA, and Piston DW (1995)
> Quantitative imaging of green fluorescent protein in cultured cells:
> comparison of microscopic techniques, use in fusion proteins and detection
> limits.  J.Microsc 180(2):109-116.
> 
> Estimate is 1um for cytoplasmic wt GFP.  Similar if GFP is part of a
> fusion protein.
> 

The detection limits should be much lower for the brighter chromophore 
variants of GFP.

-= Paul =- 

.~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~.
! Paul Kitts                                                !
! CLONTECH Laboratories, Inc.                               !
! 1020 East Meadow Circle       Email: pakitts at clontech.com !
! Palo Alto                     Phone: 415 424 8222 x1463   !
! CA 94303-4230, U.S.A.         Fax:   415 424 1064         !
 ~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~



More information about the Fluorpro mailing list

Send comments to us at biosci-help [At] net.bio.net